By A H Rebar; et al
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Additional info for A Guide to Hematology in Dogs and Cats
Haemobartonella felis. RBC density on the smear is reduced due to severe anemia. In this area of the smear, Haemobartonella organisms can be seen as basophilic rings on the RBC surface. These parasites produce hemolytic anemia by causing RBCs to be phagocytozed by macrophages (100x). Figure 4-32 Feline blood. Cytauxzoon felis. This is an intracellular protozoan parasite of wild and domestic cats. In domestic cats, the parasite causes a nonregenerative anemia with round, oval, or safety pin shaped basophilic parasites in RBCs.
20 TNM GuideToHema:03058 TNM text_cp 6/28/07 3:49 PM Page 21 3 LABORATORY METHODS IN HEMATOLOGY ® Platelet count – Can be performed using hemocytometer and ammonium oxalate diluent but it is difficult to achieve accuracy even among skilled medical technologists. – Platelet count can be estimated from the blood smear. 10-12 platelets per oil immersion field (100x nose piece objective) is an appropriate number in the dog and cat. In the absence of obvious clumping, fewer than 10-12 platelets per 100x field suggests thrombocytopenia and indicates the need for a quantitative platelet count.
Cells types are differentiated by fluorescence in the presence of acridine orange. Advantages ® The QBC is an efficient and economical way to screen blood samples. ® Instrument is simple to operate and relatively fast, producing results in about 7 minutes making it especially valuable for pre-anesthetic, acute care and in-office screening procedures. ® Hematocrit, hemoglobin, and white cell and platelet counts correlate well with reference methods. ® System flags abnormal or unexpected result. Limitations ® The major limitation is the inability to produce a complete differential count.